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Primers used were against Snail2, OB-Cadherin, Wnt5A, sFRP2 and the housekeeping genes ��-actin, GAPDH and 18S (sequences and conditions #links# in supplemental information). A relative standard curve was constructed for Snail2, OB-cadherin, Wnt5A, sFRP2 and the housekeeping genes using serial dilutions of their amplicons, and these standard curves were included in each run. Standards were run in
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Bill Cummins, owner of Appliance Master of Central New Jersey, achieves Master Technician rating as a Certified Appliance ProfessionalClinton, NJ – Bill Cummins is among a very select group of major appliance service technicians in the United States to be recognized as a Certified Appliance Professional with a Master Technician rating.To achieve this elite status the local
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The trial was registered with the Australian New Zealand Clinical Trials Registry (ACTR number: ACTRN12608000480381). Physical activity was measured using the SenseWear Pro3 Armband (SWA) (SenseWear, BodyMedia, Pittsburgh, PA, USA). The SWA was worn on the right upper arm and incorporated a biaxial accelerometer and physiologic sensors that provided an indication of energy expenditure. The SWA has
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